Scooby-Doo! Night of 100 Frights is a 3D^[7] platform game developed by Heavy Iron Studios and published by THQ for the PlayStation 2, GameCube, and Xbox. The game was released in May 2002 in North America and was released later that year in PAL regions. It was the first Scooby-Doo! video game on sixth-generation consoles. The PlayStation 2 version became a Greatest Hits title in May 2003.^{[8][9] [better source needed]} The game has a follow-up titled Scooby-Doo! Mystery Mayhem.

Daphne’s close friend, Holly Graham, has asked the Mystery Inc. crew to help her investigate the disappearance of her uncle, Professor Alexander Graham (Tim Conway), a genius and inventor. The five arrive at the mansion, known as Mystic Manor, where they are greeted by Holly; she recounts how Daphne has told her about their past cases before inviting the group to come inside, but Scooby and Shaggy refuse. Fred, Daphne, and Velma follow Holly inside, leaving Shaggy and Scooby outside alone. When Shaggy goes to obtain a box of Scooby Snacks he notices hanging from a gnarled tree, he pulls on one of the branches and falls into a trap door. Frightened, Scooby explores the grounds and discovers a nearby playground where he collects a monster token, receives some clues for the mystery, and eats all of the Scooby snacks. He then enters the mansion and discovers Holly locked in a room. She explains that a specter called The Mastermind appeared, kidnapping Fred, Daphne, and Velma before imprisoning her, and gives him a map of the region for getting around.